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| Bernal,Giuliano; Illanes,Andrés; Ciampi,Luigi. |
| The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. There are many examples of formulations using bacterial or fungal strains with biocontrol applications. Among them, members of the genus Bacillus are well known antibiotic producers. However, the increased capacity of antibiotic production obtained by direct mutagenesis of wild strains, has seldom been reported in the open literature. This research refers to the mutation of the A47 Bacillus strain, a plant pathogen antagonist, in order to obtain an improved strain with enhanced capacity to synthesize metabolites with antibiotic activity. The mutant strain M40 was obtained using the mutagenic agent acridine orange. The mutant strain showed a... |
| Tipo: Journal article |
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| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100008 |
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| Guzmán,Fanny; Barberis,Sonia; Illanes,Andrés. |
| Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis... |
| Tipo: Journal article |
Palavras-chave: Enzymatic synthesis; Peptides; Proteases; Solid-phase synthesis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012 |
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| Altamirano,Claudia; Illanes,Andrés; Canessa,Rossana; Becerra,Silvana. |
| Recombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium supplementation with vitamins, lipids, and specific amino acids on cell growth, t-PA production and biological functionality. Among vitamins, only biotin, folic acid, cobalamine and benzoic acid were required for improving growth and t-PA production. Lipid supplement allowed a significant increase cell concentration and t-PA specific activity and concentration, though its specific production rate decreased slightly. Medium supplementation with proline, serine and asparagine had also positive effects on cell growth. Besides, the addition of asparagine (even in the... |
| Tipo: Journal article |
Palavras-chave: Amino acid; Cholesterol; Vitamins. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100009 |
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| Illanes,Andrés. |
| Whey is a co-product of processes for the production of cheese and casein that retains most of the lactose content in milk. World production of whey is estimated around 200 million tons per year with an increase rate of about 2%/per year. Milk production is seasonal, so surplus whey is unavoidable. Traditionally, whey producers have considered it as a nuisance and strategies of whey handling have been mostly oriented to their more convenient disposal. This vision has been steadily evolving because of the upgrading potential of whey major components (lactose and whey proteins), but also because of more stringent regulations of waste disposal. Only the big cheese manufacturing companies are in the position of implementing technologies for their recovery and... |
| Tipo: Journal article |
Palavras-chave: Beta-galactosidase; Enzyme catalysis; Lactose; Oligosaccharides; Prebiotics; Whey. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600009 |
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